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Coverage of the HPV16 genome after <t>a</t> <t>multiplex</t> <t>PCR</t> approach combined with ONT sequencing on the SiHa cell line at 12.5 ng with Pool A (primers in equivalent concentration) or B (primers #13 and #8 in double quantities). The red dotted line represents a threshold sequencing depth at 10×, and the vertical black dotted lines separate the 14 fragments amplified by the multiplex PCR.
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Coverage of the HPV16 genome after <t>a</t> <t>multiplex</t> <t>PCR</t> approach combined with ONT sequencing on the SiHa cell line at 12.5 ng with Pool A (primers in equivalent concentration) or B (primers #13 and #8 in double quantities). The red dotted line represents a threshold sequencing depth at 10×, and the vertical black dotted lines separate the 14 fragments amplified by the multiplex PCR.
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pcr approach  (Illumina Inc)
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Coverage of the HPV16 genome after <t>a</t> <t>multiplex</t> <t>PCR</t> approach combined with ONT sequencing on the SiHa cell line at 12.5 ng with Pool A (primers in equivalent concentration) or B (primers #13 and #8 in double quantities). The red dotted line represents a threshold sequencing depth at 10×, and the vertical black dotted lines separate the 14 fragments amplified by the multiplex PCR.
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Coverage of the HPV16 genome after <t>a</t> <t>multiplex</t> <t>PCR</t> approach combined with ONT sequencing on the SiHa cell line at 12.5 ng with Pool A (primers in equivalent concentration) or B (primers #13 and #8 in double quantities). The red dotted line represents a threshold sequencing depth at 10×, and the vertical black dotted lines separate the 14 fragments amplified by the multiplex PCR.
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cdna synthesis kit approach  (TaKaRa)
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Coverage of the HPV16 genome after <t>a</t> <t>multiplex</t> <t>PCR</t> approach combined with ONT sequencing on the SiHa cell line at 12.5 ng with Pool A (primers in equivalent concentration) or B (primers #13 and #8 in double quantities). The red dotted line represents a threshold sequencing depth at 10×, and the vertical black dotted lines separate the 14 fragments amplified by the multiplex PCR.
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pcr approach  (National Reference Center for Legionella)
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Coverage of the HPV16 genome after <t>a</t> <t>multiplex</t> <t>PCR</t> approach combined with ONT sequencing on the SiHa cell line at 12.5 ng with Pool A (primers in equivalent concentration) or B (primers #13 and #8 in double quantities). The red dotted line represents a threshold sequencing depth at 10×, and the vertical black dotted lines separate the 14 fragments amplified by the multiplex PCR.
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pcr 2: a practical approach  (ACADEMIC PRESS INC)
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Coverage of the HPV16 genome after <t>a</t> <t>multiplex</t> <t>PCR</t> approach combined with ONT sequencing on the SiHa cell line at 12.5 ng with Pool A (primers in equivalent concentration) or B (primers #13 and #8 in double quantities). The red dotted line represents a threshold sequencing depth at 10×, and the vertical black dotted lines separate the 14 fragments amplified by the multiplex PCR.
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two-step pcr approach  (Illumina Inc)
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Illumina Inc two-step pcr approach
Coverage of the HPV16 genome after <t>a</t> <t>multiplex</t> <t>PCR</t> approach combined with ONT sequencing on the SiHa cell line at 12.5 ng with Pool A (primers in equivalent concentration) or B (primers #13 and #8 in double quantities). The red dotted line represents a threshold sequencing depth at 10×, and the vertical black dotted lines separate the 14 fragments amplified by the multiplex PCR.
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two-step pcr approach - by Bioz Stars, 2026-11
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Image Search Results


Coverage of the HPV16 genome after a multiplex PCR approach combined with ONT sequencing on the SiHa cell line at 12.5 ng with Pool A (primers in equivalent concentration) or B (primers #13 and #8 in double quantities). The red dotted line represents a threshold sequencing depth at 10×, and the vertical black dotted lines separate the 14 fragments amplified by the multiplex PCR.

Journal: Microbiology Spectrum

Article Title: An amplicon-based approach for full-genome characterization of HPV16

doi: 10.1128/spectrum.03073-24

Figure Lengend Snippet: Coverage of the HPV16 genome after a multiplex PCR approach combined with ONT sequencing on the SiHa cell line at 12.5 ng with Pool A (primers in equivalent concentration) or B (primers #13 and #8 in double quantities). The red dotted line represents a threshold sequencing depth at 10×, and the vertical black dotted lines separate the 14 fragments amplified by the multiplex PCR.

Article Snippet: We set up a multiplex PCR approach combined with Oxford Nanopore Technologies for the HPV16 full-genome characterization.

Techniques: Multiplex Assay, Sequencing, Concentration Assay, Amplification

Results of the sequencing depth ( A ) and the HPV16 genome coverage ( B ) after the multiplex PCR approach combined with ONT sequencing according to the HPV16 Ct value determined with the Allplex HPV28 assay. The coverage was determined with a depth sequencing of 25 ×.

Journal: Microbiology Spectrum

Article Title: An amplicon-based approach for full-genome characterization of HPV16

doi: 10.1128/spectrum.03073-24

Figure Lengend Snippet: Results of the sequencing depth ( A ) and the HPV16 genome coverage ( B ) after the multiplex PCR approach combined with ONT sequencing according to the HPV16 Ct value determined with the Allplex HPV28 assay. The coverage was determined with a depth sequencing of 25 ×.

Article Snippet: We set up a multiplex PCR approach combined with Oxford Nanopore Technologies for the HPV16 full-genome characterization.

Techniques: Sequencing, Multiplex Assay